|
KMID : 0043320060290100874
|
|
Archives of Pharmacal Research
2006 Volume.29 No. 10 p.874 ~ p.878
|
|
|
Effects of Methyl Gallate on Arachidonic Acid Metabolizing Enzymes: Cyclooxygenase-2 and 5-Lipoxygenase in Mouse Bone Marrow-Derived Mast Cells
|
|
Kim Se-Jong
Jin Meihua
Lee Eun-Kyung
Moon Tae-Chul
Quan Zhejiu
Yang Ju-Hye
Son Kun-Ho
Kim Kil-Ung
Son Jong-Kun
Chang Hyun-Wook
|
|
|
Abstract
|
|
|
|
Methyl gallate (MG) is a medicinal herbal product that is isolated from Paeonia lactiflora that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with an IC50 values of 17.0 μM. This compound also found inhibited the COX-2-dependent conversion of the exogenous arachidonic acid to PGD2 in a dose-dependent manner with an IC50 values of 19.0 μM, using a COX enzyme assay kit. However, at concentrations up to 80 μM, MG did not inhibit COX-2 protein expression in BMMC, indicating that MG inhibits COX-2 activity directly. Furthermore, MG consistently inhibited the production of leukotriene C4 (LTC4) in a dose dependent manner, with an IC50 value of 5.3 μM. These results demonstrate that MG has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity, which might provide the basis for novel anti-inflammatory drugs.
|
|
|
KEYWORD
|
|
|
Paeonia lactiflora, Methyl gallate, Inflammation, Cyclooxygenase-2, 5-Lipoxygenase, Bone marrow-derived mast cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|